GSM2633405: H2_cell_80; Homo sapiens; RNA-Seq - SRA

SRX2836592: GSM2633405: H2_cell_80; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 7.5M spots, 1.1G bases, 348.4Mb downloads

Submitted by: NCBI (GEO)

Study: Single-cell RNA-seq reveals differentiation hierarchy of normal human bone marrow and a distinct transcriptome signature of aneuploid cells

PRJNA387283 • SRP107391 • All experiments • All runs

show Abstracthide Abstract

In order to get a better understanding of the gene signature of aneuploid cells, we applied single cell RNA sequencing on human bone marrow cells from healthy donors and patients with bone marrow failure and cytogenetic abnormalities. We chacterized normal hemaopoiesis as binary differentiation and identified aneuploid cells from patient samples. Overall design: Single Cell RNA-seq The processed 'normalizedExpression.csv' file includes data for cells which pass the quality controls.

Sample: H2_cell_80

SAMN07146398 • SRS2210777 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: The C1 Single-cell Auto Prep System (Fluidigm) was employed to perform SMARTer (Clontech) WTA on up to 96 individual cells, according to the manufacturer’s protocols (full details available at www.fluidigm.com). WTA products were converted to Illumina sequencing libraries using Nextera XT (Illumina). Samples with single cell and bulk controls were sequenced on an Illumina HiSeq 2500 or 3000 Systems using a 75-bp paired-end sequencing strategy

Experiment attributes:

GEO Accession: GSM2633405

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 7.5M spots, 1.1G bases, 348.4Mb

Run	# of Spots	# of Bases	Size	Published
SRR5578378	7,458,024	1.1G	348.4Mb	2017-10-16

ID:: 4074465

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